Protein Method Blocking Buffers
Carbosynth LLC. HAMA blocking reagent, 1 gram
HAMA blocking reagent for use in assays specific for clinical false positive samples
Research Products International Corp PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a widely used buffer solution in the life science marketplace. It is a combination of phosphate-buffered saline (PBS) and Tween 20, a nonionic detergent.
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Research Products International Corp BSA-Tween 20 Blocking Solution in TBS, 500 Milliliters
Contains 3% BSA and 0.05% Tween 20 in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Bioworld Blotto (5%) in PBS, 1 L
Blotto in PBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in PBS
Abcam SN x 482, R-Type Ca2+ (Cav2.3) channel blocker, 100UG
MW 4495 Da, Purity >97%. SNX 482, R-Type Ca2+ (Cav2.3) channel blocker. Achieve your results faster with highly validated, pure and trusted compounds.
The product is subject to the following: Abcam Restricted Use Statement
Biotium Napsin-A(NAPSA/1238), CF740 conjugate, 0.1mg/mL
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Alkali Scientific CellPro™ BSA-Blocking Buffer, 3% in TBS, 250mL bottle
CellPro™ BSA-Blocking Buffer, 3% in TBS, 250mL bottle
Biotium Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat and anti-sheep secondary antibodies.
Molecular Devices Blocking Buffer, 5X Concentrate, 120 mL, Liquid
Concentrated 5X blocking buffer formulated for fluorescence western blot detection. Dilute to 1X for membrane blocking prior to primary antibody incubation to reduce background and improve signal-to-noise in fluorescence-based assays.
- Concentrated 5X formulation.
- Dilutes to 1X for use as a blocking solution.
- Supplied as a 120 mL bottle.
- Reduces background and improves signal-to-noise in fluorescence western blots.
- Compatible with membrane blocking prior to antibody incubations.
- For research use only; not for clinical or diagnostic use.
LICOR Intercept (TBS) Blocking Buffer (10 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, can be used for detection of phosphorylated targets, has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection.Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including: Quantitative Western Blot, Chemiluminescent Western Blots, In-Cell Western™ Assay, In-Gel Western, Protein Array, Glycoprotein Detection